Ti-Crest spinning disk microscope

Spinning disk system with 7 laser lines that allows to remove the spinning disk so that it may also be used in widefileld. In contrast to the Ti-VoX, the Ti-Crest supports all capabilities of Nikon's NIS Elements software as well as Nikon's Perfect Focus System (PFS).

Microscope

Fully motorized Nikon Ti2. Water dispenser for long term imaging with water immersion objectives available. On-stage incubation (Temperature and CO2) from oko lab.

Software

Nikon NIS-Elements

Confocal Scanning Unit

Crest X-Light V3

Confocal light source

Lumencor Celesta Light Engine
Available excitation laser lines:

• 405 nm
• 440 nm
• 488 nm
• 514 nm
• 561 nm
• 640 nm
• 730 nm

Cameras

Andor Zyla-4.2P

• 4.2 Megapixels (2048 x 2048)
• Pixel size 6.5 x 6.5μm
• FOV 18mm
• Sensor Area 13.3mm x 13.3mm
• Quantum Efficiency up to 82%
• 53 fps (USB 3.0), 100 fps (Camera Link)

Andor iXon 888 EMCCS camera

• 1 Megapixel (1024 x 1024)
• Pixel size 13 x 13μm
• Sensor Area 13.3mm x 13.3mm
• Single Photon Sensitive
• 30 fps full frame readout rate

 

Available objectives (subject to change)

• Nikon Plan Apo
  10x 0.45 λS
  DIC N1 WD 4.0
  coverslip thickness: 0.17mm


• Nikon Plan Apo VC
  20x 0.75
  DIC N2 WD 1.0
  coverslip thickness: 0.17mm


• Nikon Plan Apo λ
  60x 1.40 OIL
  DIC N2 WD 0.13
  coverslip thickness: 0.17mm
  Oil Immersion (n=1.515)


• Nikon Plan Apo λ
  100x 1.45 OIL
  DIC N2 WD 0.13
  coverslip thickness: 0.17mm
  Oil Immersion (n=1.515)


• LWD Apo λ 40
  40x 1.15 WI
  DIC N2 WD 0.16 – 0.18mm
  coverslip thickness: 0.15 – 0.19mm
  Water Immersion (n=1.33)


• Nikon Plan Apo IR
  60x 1.25 WI
  DIC N2 WD 0.59 - 0.61mm
  coverslip thickness: 0.15 – 0.19mm
  Water Immersion (n=1.33)

Bearbeiter: Christian Ackermann

Latest Revision: 2023-03-27